Nucleotides Quotes (1 quote)
Of the nucleosides from deoxyribonucleic acids, all that was known with any certainty [in the 1940s] was that they were 2-deoxy-D-ribosides of the bases adenine, guanine, thymine and cytosine and it was assumed that they were structurally analogous to the ribonucleosides. The chemistry of the nucleotidesthe phosphates of the nucleosideswas in a correspondingly primitive state. It may well be asked why the chemistry of these groups of compounds was not further advanced, particularly since we recognize today that they occupy a central place in the history of the living cell. True, their full significance was for a long time unrecognized and emerged only slowly as biochemical research got into its stride but I think a more important reason is to be found in the physical properties of compounds of the nucleotide group. As water-soluble polar compounds with no proper melting points they were extremely difficult to handle by the classic techniques of organic chemistry, and were accordingly very discouraging substances to early workers. It is surely no accident that the major advances in the field have coincided with the appearance of new experimental techniques such as paper and ion-exchange chromatography, paper electrophoresis, and countercurrent distribution, peculiarly appropriate to the compounds of this group.